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CRYOTEC | Oocyte and embryo vitrification method 🇯🇵 on Instagram: "Why do embryos shrink during vitrification? When vitrifying embryos, we need to remove water from the cell gradually to prevent hyperhydration caused by osmotic pressure changes. In ES step, water exits the cells, and cryoprotectant (CPA) enters. Water flows out from the cell faster than CPAs come inside. The embryos initially shrink, then recover as the CPA enters. In VS step, the embryos are then placed in a solution containi
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