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digital PCR, and whole transcriptome analysis. Figure 1. Better Efficiency of RNA Recovery at Single Cell Input. Norgen’s Single Cell RNA Purification Kit allows sensitive RNA extraction from as ...
RT-PCR was performed using human beta actin primers on 2 µL of the 50 µL of cytoplasmic RNA isolated from 1 million HeLa cells using Norgen's Cytoplasmic & Nuclear RNA Purification Kit.
Real-time PCR offers fast analysis of samples ... are likely to be subjected to similar variations in efficacy of the RNA extraction and reverse transcription and in degradation of RNA during ...
DNA & RNA isolation, PCR/qPCR, Qubit fluorometry, gene expression and drug development studies. Consultative services are also available.
Traditional PCR amplification is a qualitative assay ... Recently, researchers have been investigating the elimination RNA extraction prior to RT-qPCR. Normally, RNA is purified from a sample ...