Advancing single-cell research requires precise and reproducible tissue dissociation techniques. Manual methods can introduce variability, compromise cell viability and hinder downstream applications.

The mice were divided into the control group (CG), model group (MG), DGF group (DFG), DGF + siRNA group (DFSG), and siRNA group (SG). The mice were administered sterile water, DGF, and/or ...

(A) Lysates of HEK293T cells co-transfected with Flag-ERα and FRMD8 siRNA as indicated were immunoblotted. (B-C) Lysates from T47D cells transiently transfected with control or FRMD8 siRNA were ...

Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, No. 100 Haining Road, Shanghai 200080, China Department of Gastric Surgery, Cancer Hospital of ...

2) Functional genomics is used to assign a physiological role to the identified targets using cellular (in vivo siRNA) and whole-body (genetically modified mice) models. We are developing: a) ...

In this study, we detail our experience of the implementation of genomics and transcriptomics technologies in a pilot cohort of previously investigated patients with a typical clinical diagnosis ...

Skeletal muscle possesses an outstanding capacity to regenerate upon injury due to the adult muscle stem cell (MuSC) activity. This ability requires the proper balance between MuSC expansion and ...

This is an introduction to the biological mechanism of RNA interference (RNAi) and experimental application of short, interfering RNA (siRNA). The key steps of the endogenous RNAi pathway will be ...