Gene syntax also affects the behavior of genetic circuits, and contributes to unpredictable outcomes in genetic networks.

Poor expression of fusions. Check for expression of GFP fusions on SDS-PAGE. Vary concentrations of inducer to obtain roughly stoichiometric levels of expression. We find that 0–20 μM IPTG and ...

After erylysis, cells labeled with Mac-1-Pe, B220-Pe, Gr-1-Pe and CD4-Pe (Pharmingen, Heidelberg, Germany), were analyzed for GFP-expression. Dead cells (propidium iodide-positive) were excluded.

This manuscript demonstrates that Oct4 overexpression synergizes with Notch inhibition (Rbpj knockout) to promote the conversion of adult murine Müller glia (MG) into bipolar cells. These findings are ...