The DeltaVision OMX V4 enables Total Internal Reflection Fluorescence Microscopy (TIRF). This technique uses an evanescent wave to selectively excite fluorophores that are close to the coverslip. This ...

TIRF is a microscopy technique that is used to image fluorescent molecules, such as green fluorescent protein (GFP) and fluorochromes, in liquids that are adjacent to a solid with a high refractive ...

When a ray of light that is travelling through a piece of glass strikes the interface between the glass and the air, it changes direction according to Snell’s law. If the angle of incidence is less ...

A beam transformed: Optical transforms like tight focussing and total internal reflection, which gives rise to an evanescent field, can lead to out-of-plane polarization components and transverse spin ...

In TIRF microscopy, fluorescent molecules are in a sample in an aqueous environment that is near a solid with a high refractive index, usually a glass coverslip. At what is called the critical angle, ...