In RNA-seq library preparation, selecting the appropriate number of PCR cycles is a critical balancing act to avoid overcycling.
Conventional PCR systems require users to set a predetermined number of cycles based on assay- and sample-specific factors. When performing single-cell sequencing experiments, this requires knowledge ...
In this Innovation Spotlight, Marwan A. Alsarraj, global segment manager at Bio-Rad Laboratories, discusses how digital PCR eliminates the need for standard curves while achieving high sensitivity and ...
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